Mouse IL-18 R alpha/IL-1 R5 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-18 R alpha/IL-1 R5 Affinity Purified Polyclonal Ab (25 UG)

货号： AF856-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF856-SP
别名	CD218a; IL-1 R5; IL-1 Rrp; IL-18 R1; IL18R1
全称	Interleukin 18 Receptor alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. Ahn, H.J. et al. (1997) J. Immunol. 159:2125. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8809 (Human); 16174 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA J. Biol. Chem., 2009;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs. Authors: Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A Int. Immunol., 2008;20(12):1565-73. Species: Mouse Sample Type: Whole Cells Application: Flow Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. Authors: Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A J. Immunol., 2008;180(4):2322-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation. Authors: Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ J. Biol. Chem., 2007;283(7):4200-9. Species: Mouse Sample Type: Whole Cells Application: Flow Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation. Authors: Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B Am. J. Pathol., 2006;169(3):967-76. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Authors: Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Cancer Res., 2006;66(21):10497-504. Species: Mouse Sample Type: Whole Cells Application: Flow Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP J. Immunol., 2004;172(3):1754-62. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5 Ser19-Gly326 Accession # Q61098
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Angiogenesis gamma delta T Cells Human CD Marker Products ICC / IHC Images: IL-18 R alpha/IL-1 R5 IL-1 Family Inflammasomes: Intracellular Regulators of Pathogen Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells Mini-review: Interleukin 18 (IL-18) Neuroinflammation T Cell Cytokine Signaling Th1 Cells

参考图片

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Mouse IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND₅₀ is typically 0.2-0.8 µg/mL.