Mouse IGF-II Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IGF-II Affinity Purified Polyclonal Ab (25 UG)

货号： AF792-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF792-SP
别名	C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; IGF2; IGF-2; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PP9974; somatomedin-A
全称	Insulin-like Growth Factor II
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IGF-II in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IGF‑II is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IGF‑II-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse IGF‑II.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3481 (Human); 16002 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation. Authors: Ostrovsky O, Ahmed NT, Argon Y Mol. Biol. Cell, 2009;20(6):1855-64. Species: Mouse Sample Type: Cell Lysates Application: WB Role of IGF signaling in olfactory sensory map formation and axon guidance. Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J Neuron, 2008;57(6):847-57. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Authors: Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y Mol. Biol. Cell, 2007;18(10):3764-75. Species: Mouse Sample Type: Tissue Homogenates Application: WB Hypoxia-independent overexpression of hypoxia-inducible factor 1alpha as an early change in mouse hepatocarcinogenesis. Authors: Tanaka H, Yamamoto M, Hashimoto N, Miyakoshi M, Tamakawa S, Yoshie M, Tokusashi Y, Yokoyama K, Yaginuma Y, Ogawa K Cancer Res., 2006;66(23):11263-70. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IGF-II Ala25-Glu91 Accession # P09535
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1‑3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In humans, IGF-II is primarily synthesized by the liver and circulates at high levels in both fetus and adult. In rodents, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited or local effect only in rodents. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11), plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112). This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15). There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor. This is distinct from IGF-II, is secreted by pancreatic B cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners. It binds to IGF-I R, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type II IGF receptor (IGF-II R), and IGF binding proteins 1-6 (18, 19). The first three receptors initiate downstream signaling events, the IGF-II R sequesters local IGF‑II, and the six IGFBPs regulate IGF-II activity in various tissues.
运输条件	Blue Ice
存放说明	4℃
参考文献	LeRoith, D. and C.T. Roberts Jr. (2003) Cancer Lett. 195:127. Werner, H. and D. LeRoith (2000) Cell. Mol. Life Sci. 57:932. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687. Rotwein, P. and L.J. Hall (1990) DNA Cell Biol. 10:725. Chao, W. and P.A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265. Goya, L. et al. (1999) J. Biol. Chem. 274:24633. McCarthy, T.L. et al. (1992) Endocrinology 130:1303. Zindy, F. et al. (1992) J. Hepatol. 14:30. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65. Stempien, M.M. et al. (1986) DNA 5:357. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823. Buchanan, C.M. et al. (2001) Biochem. J. 360:431. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.

参考图片

IGF-II in Mouse Embryo. IGF-II was detected in immersion fixed frozen sections of mouse embryo (E13-17) using Mouse IGF-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by IGF‑II and Neutralization by Mouse IGF‑II Antibody. Recombinant Mouse IGF‑II (Catalog # 792-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF‑II (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792). The ND₅₀ is typically 0.5-2.5 µg/mL.