Mouse IGF-I Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IGF-I Affinity Purified Polyclonal Ab (25 UG)

货号： AF791-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF791-SP
别名	IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
全称	Insulin-like Growth Factor I
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IGF‑I in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat IGF-I is observed, 15% cross-reactivity with recombinant human IGF-I is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF‑I.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling. Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B J Neurosci, 2014;34(38):12788-800. Species: Rat Sample Type: In Vivo Application: In Vivo Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9. Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M J. Neurochem., 2009;111(6):1409-24. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Role of IGF signaling in olfactory sensory map formation and axon guidance. Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J Neuron, 2008;57(6):847-57. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat. Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y Biochem. Biophys. Res. Commun., 2008;368(1):81-7. Species: Mouse Sample Type: In Vivo Application: In vivo Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells. Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M Mol. Cell. Neurosci., 2006;31(1):149-60. Species: Mouse Sample Type: Whole Cells Application: ICC Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M J. Clin. Invest., 2006;116(4):905-15. Species: Mouse Sample Type: Whole Cells Application: ICC Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1. Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11784-9. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IGF-I Gl33-Ala102 Accession # Q8CAR0
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Philippou, A. et al. (2007) In Vivo 21:45. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275. Malemud, C.J. (2007) Clin. Chim. Acta 375:10. Samani, A.A. et al. (2007) Endocrine Rev. 28:20. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

参考图片

Cell Proliferation Induced by IGF‑I and Neutralization by Mouse IGF‑I Antibody. Recombinant Mouse IGF‑I (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF‑I (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND₅₀ is typically 0.2-1.0 µg/mL.

IGF‑I in Mouse Embryo. IGF‑I was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF‑I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.