Mouse CCL27/CTACK Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL27/CTACK Affinity Purified Polyclonal Ab (25 UG)

货号： AF725-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF725-SP
别名	ALP; C-C motif chemokine 27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CCL27/CTACK in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL27/CTACK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL27/CTACK.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10850 (Human); 20301 (Mouse); 362505 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. Authors: Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM Infect. Immun., 2005;73(7):3896-902. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL27/CTACK Leu26-Asn120 Accession # NP_035466
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kunkel, L. and E.C. Butcher (2002) Immunity 16:1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737. Gortz, A. et al. (2002) J. Immunol. 169:1387. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96:14470. Homey, B. et al. (2000) J. Immunol. 164:3465. Homey, B. et al. (2002) Nat. Med. 8:157. Inokuma, D. et al. (2006) Stem Cells 24:2810. Jarmin, D. et al. (2000) J. Immunol. 164:3460. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541. Soler, D. et al. (2003) Blood 101:1677. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

参考图片

Chemotaxis Induced by CCL27/CTACK and Neutralization by Mouse CCL27/
CTACK Antibody. Recombinant Mouse CCL27/CTACK (Catalog # 725‑CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Alamar blue staining. Chemotaxis elicited by Recombinant Mouse CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF725). The ND₅₀ is typically
10‑20 µg/mL.

CCL27/CTACK in Mouse Skin. CCL27/CTACK was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.