Mouse VEGF R2/Flk-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse VEGF R2/Flk-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF644-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF644-SP
别名	CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGFR; VEGFR2; VEGFR-2
全称	Vascular Endothelial Growth Factor Receptor 2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse VEGF R2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGF R2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGF R1 and rmVEGF R3 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 50 ng/mL Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF₁₆₄.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins Authors: MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen Genesis, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli Sci Rep, 2015;5(0):7890. Species: Rat Sample Type: Whole Cells Application: Bioassay Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C J Clin Invest, 2015;125(7):2661-76. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma. Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L Cancer Res, 2013;73(24):7254-64. Species: Human Sample Type: Tissue Lysate Application: WB The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy. Authors: Schreurs MP, Houston EM, May V, Cipolla MJ FASEB J., 2012;26(1):355-62. Species: Rat Sample Type: In Vivo Application: In vivo VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L J. Exp. Med., 2012;209(7):1363-77. Species: Mouse Sample Type: Tissue Homogenates Application: WB Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Nature, 2012;484(7392):110-4. Species: Mouse Sample Type: Cell LysatesHEK Application: ELISA Development Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy. Authors: Efe JA, Hilcove S, Kim J, Zhou H, Ouyang K, Wang G, Chen J, Ding S Nat. Cell Biol., 2011;13(3):215-22. Species: Mouse Sample Type: Whole Cells Application: ICC Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Authors: Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A Nature, 2010;465(7297):487-91. Species: Mouse Sample Type: Cell Lysates Application: IP Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Authors: Lohela M, Helotera H, Haiko P, Dumont DJ, Alitalo K Am. J. Pathol., 2008;173(6):1891-901. Species: Mouse Sample Type: Whole Tissue Application: IHC Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K Nature, 2008;454(7204):656-60. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Authors: Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N Exp. Hematol., 2008;36(5):545-558. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Cancer Res., 2007;67(12):5789-97. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors. Authors: Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L Stem Cells, 2007;25(12):2987-95. Species: Mouse Sample Type: Cell Lysates Application: IP Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting. Authors: Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, Nagy JA, Dvorak HF, Yla-Herttuala S, Shibuya M, Alitalo K J. Exp. Med., 2007;204(6):1431-40. Species: Mouse Sample Type: Whole Tissue Application: IHC Chronic hydrocephalus-induced hypoxia: increased expression of VEGFR-2+ and blood vessel density in hippocampus. Authors: Dombrowski SM, Deshpande A, Dingwall C, Leichliter A, Leibson Z, Luciano MG Neuroscience, 2007;152(2):346-59. Species: Canine Sample Type: Whole Tissue Application: IHC Frozen Anthrax lethal toxin inhibits growth of and vascular endothelial growth factor release from endothelial cells expressing the human herpes virus 8 viral G protein coupled receptor. Authors: Depeille P, Young JJ, Boguslawski EA, Berghuis BD, Kort EJ, Resau JH, Frankel AE, Duesbery NS Clin. Cancer Res., 2007;13(19):5926-34. Species: Mouse Sample Type: Whole Cells Application: ICC Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2. Authors: Yamaji-Kegan K, Su Q, Angelini DJ, Champion HC, Johns RA Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(6):L1159-68. Species: Mouse Sample Type: In Vivo Application: In vivo Increased VEGF levels induced by anti-VEGF treatment are independent of tumor burden in colorectal carcinomas in mice. Authors: Schmitz V, Vilanueva H, Raskopf E, Hilbert T, Barajas M, Dzienisowicz C, Gorschluter M, Strehl J, Rabe C, Sauerbruch T, Prieto J, Caselmann WH, Qian C Gene Ther., 2006;13(16):1198-205. Species: Mouse Sample Type: Serum Application: WB Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Chem. Biol., 2006;13(5):549-56. Species: Human Sample Type: Whole Cells Application: Flow Inhibition of prostate tumor growth and bone remodeling by the vascular targeting agent VEGF121/rGel. Authors: Mohamedali KA, Poblenz AT, Sikes CR, Navone NM, Thorpe PE, Darnay BG, Rosenblum MG Cancer Res., 2006;66(22):10919-28. Species: Mouse Sample Type: Whole Cells Application: Neut Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A. Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J J. Clin. Invest., 2006;116(2):422-9. Species: Mouse Sample Type: Cell Lysates Application: IP Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue. Authors: Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK J. Lab. Clin. Med., 2005;145(4):194-203. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP J. Immunol., 2005;174(1):215-22. Species: Mouse Sample Type: Cell Lysates Application: WB Effective angiostatic treatment in a murine metastatic and orthotopic hepatoma model. Authors: Raskopf E, Dzienisowicz C, Hilbert T, Rabe C, Leifeld L, Wernert N, Sauerbruch T, Prieto J, Qian C, Caselmann WH, Schmitz V Hepatology, 2005;41(6):1233-40. Species: Mouse Sample Type: Serum Application: ELISA Development All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E Leuk. Res., 2004;28(3):243-8. Species: Human Sample Type: Whole Cells Application: ICC Vascular endothelial growth factor-B promotes in vivo angiogenesis. Authors: Silvestre JS, Tamarat R, Ebrahimian TG, Le-Roux A, Clergue M, Emmanuel F, Duriez M, Schwartz B, Branellec D, Levy BI Circ. Res., 2003;93(2):114-23. Species: Mouse Sample Type: In Vivo Application: In vivo VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. Authors: Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C J. Cell Biol., 2003;161(6):1163-77. Species: Rat Sample Type: In Vivo Application: In vivo Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction. Authors: Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T Blood, 2002;100(7):2487-93. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2 Ala20-Glu762 Accession # P35918
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

参考图片

VEGF R2/KDR/Flk‑1 in Mouse Embryo. VEGF R2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

VEGF R2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGF R2/KDR/Flk‑1 Antibody. Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF₁₆₄(Catalog #493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF₁₆₄ (5 ng/mL) activity elicited by Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND₅₀ is typically 0.1-0.3 µg/mL.