| 货号 | AF582-SP |
| 别名 | Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 | 全称 | Interleukin 22 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(1-4 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse IL-22 in ELISAs and Western blots. In sandwich immunoassays, approximately 75% cross-reactivity with recombinant rat IL‑22 is observed and less than 25% cross-reactivity with recombinant human IL-22 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 1-4 µg/mL ELISA Detection (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑22. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50616 (Human); 50929 (Mouse); 500836 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse IL-22 Leu34-Val179 Accession # Q9JJY9 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
IL‑10 Secretion Induced by IL‑22 and Neutralization by Mouse IL‑22 Antibody. Recombinant Mouse IL‑22 (Catalog # 582-ML) stimulates IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ELISA Development Kit (Catalog # DY217B). IL‑10 secretion elicited by Recombinant Mouse IL‑22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND50 is typically 0.5-2.5 µg/mL. |
