Mouse CCL28 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL28 Affinity Purified Polyclonal Ab (25 UG)

货号： AF533-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF533-SP
别名	CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse CCL28 in direct ELISAs and Western blots.In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CCL28 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 5-25 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56477 (Human); 56838 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. Authors: Cha HR, Ko HJ, Kim ED J. Immunol., 2011;187(6):3044-52. Species: Mouse Sample Type: Tissue Homogenates Application: WB Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination. Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN J. Immunol., 2009;182(11):6851-60. Species: Mouse Sample Type: In Vivo Application: In vivo CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells. Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O J. Immunol., 2004;173(6):3668-75. Species: Mouse Sample Type: Whole Cells Application: Neut CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O J. Immunol., 2003;170(3):1452-61. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL28 Ile23-Arg130 Accession # Q9JIL2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wang, W.et al. (2000) J. Biol. Chem. 275:22313.

参考图片

Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF533). The ND₅₀ is typically 5-25 µg/mL.