Mouse HGF R/c-MET Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse HGF R/c-MET Affinity Purified Polyclonal Ab (25 UG)

货号： AF527-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF527-SP
别名	AUTS9; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
全称	Hepatocyte Growth Factor Receptor
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 256-GF) to immobilized Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527‑ME) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4233 (Human); 17295 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and Hearing J Neurosci, 2016;36(31):8200-9. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes Authors: Rachel Barrow-McG Nat Commun, 2016;7(0):11942. Species: Mouse Sample Type: Cell Lysates Application: WB Receptor Tyrosine Kinase Met Promotes Cell Survival via Kinase-Independent Maintenance of Integrin ?3?1 Authors: Lia Tesfay Mol Biol Cell, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Nat Commun, 2015;6(0):6776. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G PLoS ONE, 2015;10(5):e0125797. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded MET is required for the recruitment of anti-tumoural neutrophils. Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M Nature, 2015;522(7556):349-53. Species: Mouse Sample Type: Cell Lysates Application: ELISA Capture Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter. Authors: Wei W, Howard P, Macarak E BMC Urol, 2013;13(0):62. Species: Mouse Sample Type: tissue Application: IHC Frozen Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling. Authors: Tesio M, Golan K, Corso S Blood, 2011;117(2):419-28. Species: Mouse Sample Type: Cell Lysates Application: WB Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis. Authors: Moransard M, Sawitzky M, Fontana A, Suter T Glia, 2010;58(5):559-71. Species: Mouse Sample Type: Cell Lysates Application: IP c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74. Authors: Gordin M, Tesio M, Cohen S J. Immunol., 2010;185(4):2020-31. Species: Mouse Sample Type: Cell Lysates Application: WB High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Authors: Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE Am. J. Physiol., Cell Physiol., 2010;298(3):C465-76. Species: Rat Sample Type: Whole Cells Application: ICC Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation. Authors: Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP, Ikeuchi Y, Allen RE Am. J. Physiol., Cell Physiol., 2009;297(2):C238-52. Species: Rat Sample Type: Whole Cells Application: ICC Purification and characterization of mouse lacrimal gland epithelial cells and reconstruction of an acinarlike structure in three-dimensional culture. Authors: Ueda Y, Karasawa Y, Satoh Y, Nishikawa S, Imaki J, Ito M Invest. Ophthalmol. Vis. Sci., 2009;50(5):1978-87. Species: Mouse Sample Type: Whole Cells Application: ICC A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation. Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9. Species: Rat Sample Type: Cell Culture Supernates Application: ELISA Development A selective small molecule inhibitor of c-Met, PHA-665752, reverses lung premalignancy induced by mutant K-ras. Authors: Yang Y, Wislez M, Fujimoto N, Prudkin L, Izzo JG, Uno F, Ji L, Hanna AE, Langley RR, Liu D, Johnson FM, Wistuba I, Kurie JM Mol. Cancer Ther., 2008;7(4):952-60. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Ets-1 triggers and orchestrates the malignant phenotype of mammary cancer cells within their matrix environment. Authors: Furlan A, Vercamer C, Desbiens X, Pourtier A J. Cell. Physiol., 2008;215(3):782-93. Species: Mouse Sample Type: Whole Cells Application: Neut Gastrointestinal hormones cause rapid c-Met receptor down-regulation by a novel mechanism involving clathrin-mediated endocytosis and a lysosome-dependent mechanism. Authors: Hoffmann KM, Tapia JA, Berna MJ, Thill M, Braunschweig T, Mantey SA, Moody TW, Jensen RT J. Biol. Chem., 2006;281(49):37705-19. Species: Mouse Sample Type: Whole Cells Application: ICC A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells. Authors: Lai L, Zeff RA, Goldschneider I Blood, 2005;107(5):1776-84. Species: Mouse Sample Type: Cell Lysates Application: WB Metastatic squamous cell carcinoma cells that overexpress c-Met exhibit enhanced angiogenesis factor expression, scattering and metastasis in response to hepatocyte growth factor. Authors: Dong G, Lee TL, Yeh NT, Geoghegan J, Van Waes C Oncogene, 2004;23(37):6199-208. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-MET Glu25-Asn929 Accession # P16056
内毒素水平	<0.01 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11-18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11-18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915. Corso, S. et al. (2005) Trends Mol. Med. 11:284. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039. Chan, A.M. et al. (1988) Oncogene 2:593. Lee, C.-C. and K.M. Yamada (1994) J. Biol. Chem. 269:19457. Lee, C.-C., et al. (1995) J. Biol. Chem. 270:507. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793. Ponzetto, C. et al. (1994) Cell 77:261. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408. Jo, M. et al. (2000) J. Biol. Chem. 275:8806. Wang, X. et al. (2002) Mol. Cell 9:411. Trusolino, L. et al. (2001) Cell 107:643. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720. Conrotto, P. et al. (2004) Oncogene 23:5131. Follenzi, A. et al. (2000) Oncogene 19:3041. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.

参考图片

HGF R/c‑MET in Mouse Embryo. HGF R/c‑MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.