Mouse IL-11 R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-11 R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF490-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF490-SP
别名	AI314697; GP130; IL11RA; IL-11RA; Il11ra2; NR1
全称	Interleukin 11 Receptor alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse IL-11 R alpha in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND₅₀) is typically 2-6 µg/mL in the presence of 0.3 ng/mL Recombinant Mouse IL‑11.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3590 (Human); 16157 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR Cell. Signal., 2009;21(1):151-60. Species: Rat Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-11 R alpha Ser22-Gln367 Accession # Q64385
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse interleukin 11 receptor alpha (IL-11 R alpha ), originally designated NR1, is a member of the hematopoietic cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 432 amino acid (aa) residue precursor type I membrane protein with a 23 aa residue signal peptide, a 344 aa residue extracellular domain containing two potential glycosylation sites, a 26 aa residue transmembrane region and a short (39 aa residue) cytoplasmic domain. In comparison with other hematopoietic cytokine receptors, the extracellular domain of IL-11 R alpha is closely related to that of IL-6 R alpha, CNTF R alpha and the p40 subunit of IL‑12, exhibiting 24%, 22% and 16% amino acid sequence identity, respectively. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	Taga, T. and T. Kishimoto (1997) Annu. Rev. Immuno. 15:797. Hilton, D. et al. (1994) EMBO J. 13:4765. Davidson, A.J. et al. (1997) Stem Cells 15:119. Curtis, D.J. et al. (1997) Blood 90:4403.

参考图片

Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody.

Recombinant Mouse IL‑11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑11 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑11 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF490). The ND₅₀ is typically 2‑6 µg/mL.