| 货号 | AF487-SP |
| 别名 | C10; MRP-1; SCYA6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects CCL6/C10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant mouse MIP-1 gamma is observed and less than 5% cross-reactivity with recombinant human MIP-1δ is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL6/C10. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20305 (Mouse); 287910 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive assessment of chemokine expression profiles by flow cytometry. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CCL6/C10 Gly22-Ala116 Accession # P27784 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL6/C10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF487). The ND50 is typically 2‑10 µg/mL. |
