| 货号 | AF-481-SP |
| 别名 | C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine |
| 反应种属 | Mouse |
| 应用 | Western Blot,Immunohistochemistry,Neutralization |
| 目标/特异性 | Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TECK is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. 55 μg/mL of this antibody will neutralize 1.6 μg/mL of recombinant mouse TECK‑induced chemotactic effect on BaF/3 hCCR9 cells by approximately 80‑90%. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6370 (Human); 20300 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CCL25/TECK Gln24-Asn144 Accession # O35903 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Human CCL25 cDNA has also been cloned and shown to encode a 151 amino acid protein that exhibits 49% amino acid sequence identity to mouse CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/ TECK Antibody. Recombinant Mouse CCL25/TECK (Catalog # 481‑TK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL25/TECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑481‑NA). 55 μg/mL of this antibody will neutralize rmTECK-induced chemotactic effect on BaF/3 hCCR9 cells by approximately 80‑90%. |
