Mouse CCL5/RANTES Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL5/RANTES Affinity Purified Polyclonal Ab (25 UG)

货号： AF478-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF478-SP
别名	chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) CCL5/RANTES and recombinant cotton rat CCL5/RANTES is observed, and less than 3% cross-reactivity with rhCCL3/MIP-1 alpha and recombinant mouse CCL3/MIP-1 alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.07-0.3 µg/mL in the presence of 0.05 µg/mL Recombinant Mouse CCL5/RANTES.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease J Biol Chem, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: Neut Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. Authors: Herz J, Johnson K, McGavern D J Exp Med, 2015;212(8):1153-69. Species: Mouse Sample Type: Whole Cells Application: Flow Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes. Authors: Laubli H, Spanaus KS, Borsig L Blood, 2009;114(20):4583-91. Species: Mouse Sample Type: Whole Tissue Application: IHC CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N J. Immunol., 2008;181(9):6384-93. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes. Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y J. Neurosci., 2008;28(13):3277-90. Species: Mouse Sample Type: Whole Cells Application: ICC TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus. Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C J. Immunol., 2007;179(11):7949-58. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Viral infection of the lungs through the eye. Authors: Bitko V, Musiyenko A, Barik S J. Virol., 2007;81(2):783-90. Species: Mouse Sample Type: Tissue Homogenates Application: WB A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A Blood, 2005;107(5):2112-22. Species: Mouse Sample Type: Whole Tissue Application: IHC CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Authors: Cardona AE, Gonzalez PA, Teale JM Infect. Immun., 2003;71(5):2634-42. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL5/RANTES
内毒素水平	<0.30 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).
运输条件	Blue Ice
存放说明	4℃
参考文献	Schall, T.J. et al. (1990) Nature 347:669. Bacon, K.B. et al. (1995) Science 269:1727. Fischer, F.R. et al. (2001) J. Immunol. 167:1637. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477. Heeger, P. et al. (1992) Kidney Int. 41:220. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83. Levy, J.A. (2009) J. Immunol. 182:3945. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401. Proost, P. et al. (1998) J. Biol. Chem. 273:7222. Appay, V. et al. (1999) J. Biol. Chem. 274:27505. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885. von Hundelshausen, P. et al. (2005) Blood 105:924. von Hundelshausen, P. et al. (2001) Circulation 103:1772. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897. Baltus, T. et al. (2003) Blood 102:1985. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

参考图片

Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478‑MR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/
RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND₅₀ is typically 0.07-0.3 µg/mL.

CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.