Mouse CX3CL1/Fractalkine Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse CX3CL1/Fractalkine Affinity Purified Polyclonal Ab (25 UG)

货号： AF472-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF472-SP
别名	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse Fractalkine in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Fractalkine and recombinant rat Fractalkine, 20% cross-reactivity with rhMCP-1, and 10% cross-reactivity with rhEotaxin is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow Authors: Yoh Goto Stem Cells, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Interaction between CX3CL1 and CX3CR1 regulates vasculitis induced by immune complex deposition. Authors: Morimura S, Sugaya M, Sato S Am J Pathol, 2013;182(5):1640-7. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis. Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T J. Immunol., 2008;181(6):4208-18. Species: Mouse Sample Type: Whole Cells Application: ICC Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function. Authors: Ishida Y, Gao JL, Murphy PM J. Immunol., 2008;180(1):569-79. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects. Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K J. Leukoc. Biol., 2007;81(1):176-85. Species: Mouse Sample Type: Whole Tissue Application: IHC Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice. Authors: Ren T, Chen Q, Tian Z, Wei H Biochem. Biophys. Res. Commun., 2007;364(4):978-84. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CX3CL1/Fractalkine Leu22-Lys105 Accession # O35188
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CX3C Chemokines and Receptors Fractalkine in Glomerulonephritis Fractalkine Signaling Modulates Neurotoxicity in vivo Glomerulonephritis: Beyond Inflammation ICC / IHC Images: CX3CL1/Fractalkine Macrophage Activation Markers Neuroinflammation Renal Markers Special Glycoproteins

参考图片

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND₅₀ is typically 0.3-1.5 µg/mL.