Mouse VEGF R1/Flt-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse VEGF R1/Flt-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF471-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF471-SP
别名	EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGFR1; VEGFR-1
全称	Vascular Endothelial Growth Factor Receptor 1
反应种属	Mouse
应用	Western Blot,Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse VEGF R1/Flt-1 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant human VEGF R1 is observed, approximately 4% cross-reactivity with recombinant mouse (rm) VEGF₁₆₄ is observed, less than 1% cross-reactivity with recombinant rat VEGF₁₆₄ is observed, and less than 0.2% cross-reactivity with rmVEGF R2 and rmVEGF R3 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse PlGF-2 (Catalog # 465-PL) to immobilized Recombinant Mouse VEGF R1/Flt-1 Fc Chimera (Catalog # 471-F1) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding. ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize VEGF₁₆₄-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 5 ng/mL Recombinant Mouse VEGF₁₆₄.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli Sci Rep, 2015;5(0):7890. Species: Rat Sample Type: Whole Cells Application: Bioassay Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Nature, 2012;484(7392):110-4. Species: Mouse Sample Type: Tissue Lysate Application: IP The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy. Authors: Schreurs MP, Houston EM, May V, Cipolla MJ FASEB J., 2012;26(1):355-62. Species: Rat Sample Type: In Vivo Application: In vivo Hypoxia-inducible factor-2alpha regulates GM-CSF-derived soluble vascular endothelial growth factor receptor 1 production from macrophages and inhibits tumor growth and angiogenesis. Authors: Roda JM, Sumner LA, Evans R, Phillips GS, Marsh CB, Eubank TD J. Immunol., 2011;187(4):1970-6. Species: Mouse Sample Type: In Vivo Application: In vivo Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells. Authors: Shin JY, Yoon IH, Kim JS, Kim B, Park CG Cell. Immunol., 2009;256(1):72-8. Species: Mouse Sample Type: Whole Cells Application: Neut Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages. Authors: Eubank TD, Roberts RD, Khan M, Curry JM, Nuovo GJ, Kuppusamy P, Marsh CB Cancer Res., 2009;69(5):2133-40. Species: Mouse Sample Type: In Vivo Application: In vivo VEGF promotes vascular sympathetic innervation. Authors: Marko SB, Damon DH Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52. Species: Rat Sample Type: Whole Cells Application: ICC VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. Authors: Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, Lennartsson J, Zhu C, Qu Y, Fang C, Hua J, Matsuo O, Fong GH, Ding H, Cao Y, Becker KG, Nash A, Heldin CH, Li X J. Clin. Invest., 2008;118(3):913-23. Species: Mouse Sample Type: In Vivo Application: In vivo alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner. Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM Blood, 2007;111(4):1980-8. Species: Mouse Sample Type: Cell Lysates Application: WB Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Am. J. Pathol., 2006;169(4):1167-82. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL Nat. Neurosci., 2006;9(3):340-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Inhibition of prostate tumor growth and bone remodeling by the vascular targeting agent VEGF121/rGel. Authors: Mohamedali KA, Poblenz AT, Sikes CR, Navone NM, Thorpe PE, Darnay BG, Rosenblum MG Cancer Res., 2006;66(22):10919-28. Species: Mouse Sample Type: Whole Cells Application: Neut Corneal avascularity is due to soluble VEGF receptor-1. Authors: Ambati BK, Nozaki M, Singh N, Takeda A, Jani PD, Suthar T, Albuquerque RJ, Richter E, Sakurai E, Newcomb MT, Kleinman ME, Caldwell RB, Lin Q, Ogura Y, Orecchia A, Samuelson DA, Agnew DW, St Leger J, Green WR, Mahasreshti PJ, Curiel DT, Kwan D, Marsh H, Ikeda S, Leiper LJ, Collinson JM, Bogdanovich S, Khurana TS, Shibuya M, Baldwin ME, Ferrara N, Gerber HP, De Falco S, Witta J, Baffi JZ, Raisler BJ, Ambati J Nature, 2006;443(7114):993-7. Species: Mouse Sample Type: In Vivo Application: In vivo Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A. Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J J. Clin. Invest., 2006;116(2):422-9. Species: Mouse Sample Type: Cell Lysates Application: IP Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP J. Immunol., 2005;174(1):215-22. Species: Mouse Sample Type: Cell Lysates Application: WB Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue. Authors: Gudehithlu KP, Ahmed N, Wu H, Litbarg NO, Garber SL, Arruda JA, Dunea G, Singh AK J. Lab. Clin. Med., 2005;145(4):194-203. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Authors: Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP Invest. Ophthalmol. Vis. Sci., 2004;45(2):368-74. Species: Mouse Sample Type: In Vivo Application: In vivo Vascular endothelial growth factor-B promotes in vivo angiogenesis. Authors: Silvestre JS, Tamarat R, Ebrahimian TG, Le-Roux A, Clergue M, Emmanuel F, Duriez M, Schwartz B, Branellec D, Levy BI Circ. Res., 2003;93(2):114-23. Species: Mouse Sample Type: In Vivo Application: In vivo VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. Authors: Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, Jeltsch M, Mitchell C, Alitalo K, Shima D, Betsholtz C J. Cell Biol., 2003;161(6):1163-77. Species: Rat Sample Type: In Vivo Application: In vivo Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction. Authors: Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T Blood, 2002;100(7):2487-93. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt-1 Ser27-Glu759 Accession # P35969
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	He, Y.et al. (1999) Molecular Endocrinology 13:537. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

参考图片

Cell Proliferation Induced by VEGF₁₆₄ and Neutralization by Mouse VEGF R1/Flt‑1 Antibody. Recombinant Mouse VEGF₁₆₄(Catalog #493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF₁₆₄ (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND₅₀ is typically 2-8 µg/mL.