Mouse CCL21/6Ckine Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL21/6Ckine Affinity Purified Polyclonal Ab (25 UG)

货号： AF457-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF457-SP
别名	6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize CCL21/6Ckine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL21/6Ckine.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6366 (Human); 18829 (Mouse); 298006 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration Authors: C Colin Brinkman Nat Commun, 2016;7(0):12021. Species: Mouse Sample Type: Whole Tissue Application: Functional Assay Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells. Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G J Immunol, 2015;194(11):5200-10. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Structural and functional features of central nervous system lymphatic vessels. Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J Nature, 2015;523(7560):337-41. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified TWEAK promotes peritoneal inflammation. Authors: Sanz A, Aroeira L, Bellon T, del Peso G, Jimenez-Heffernan J, Santamaria B, Sanchez-Nino M, Blanco-Colio L, Lopez-Cabrera M, Ruiz-Ortega M, Egido J, Selgas R, Ortiz A PLoS ONE, 2014;9(3):e90399. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F Clin Cancer Res, 2014;20(8):2147-58. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D Am J Pathol, 2014;184(5):1577-92. Species: Mouse Sample Type: Whole Tissue Application: IHC Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli. Authors: Betterman K, Paquet-Fifield S, Asselin-Labat M, Visvader J, Butler L, Stacker S, Achen M, Harvey N Am J Pathol, 2012;181(6):2225-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Exposure to Ionizing Radiation Induces the Migration of Cutaneous Dendritic Cells by a CCR7-Dependent Mechanism. Authors: Cummings, Ryan J, Gerber, Scott A, Judge, Jennifer, Ryan, Julie L, Pentland, Alice P, Lord, Edith M J Immunol, 2012;189(9):4247-57. Species: Mouse Sample Type: Whole Tissue Application: IHC NK cells regulate CD8+ T cell priming and dendritic cell migration during influenza A infection by IFN-gamma and perforin-dependent mechanisms. J Immunol, 2012;189(5):2099-109. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Authors: Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, Buchner G, Dorken B, Lipp M, Hopken UE Blood, 2011;118(4):1020-33. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Induction of targeted cell migration by cutaneous administration of a DNA vector encoding a biologically active chemokine CCL21. Authors: Jalili A, Pashenkov M, Kriehuber E, Wagner C, Nakano H, Stingl G, Wagner SN J. Invest. Dermatol., 2010;130(6):1611-23. Species: Mouse Sample Type: Cell Lysates Application: WB Ontogeny of stromal organizer cells during lymph node development. Authors: Benezech C, White A, Mader E, Serre K, Parnell S, Pfeffer K, Ware CF, Anderson G, Caamano JH J. Immunol., 2010;184(8):4521-30. Species: Mouse Sample Type: Whole Cells Application: ICC Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination. Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN J. Immunol., 2009;182(11):6851-60. Species: Mouse Sample Type: In Vivo Application: In vivo Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines. Authors: St John AL, Abraham SN Nat. Med., 2009;15(11):1259-65. Species: Mouse Sample Type: In Vivo Application: In vivo Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice. Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ J. Exp. Med., 2009;206(1):233-48. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Macrophage mannose receptor on lymphatics controls cell trafficking. Authors: Marttila-Ichihara F, Turja R, Miiluniemi M, Karikoski M, Maksimow M, Niemela J, Martinez-Pomares L, Salmi M, Jalkanen S Blood, 2008;112(1):64-72. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The chemokine receptor CCR7 mediates corneal antigen-presenting cell trafficking. Authors: Jin Y, Shen L, Chong EM Mol. Vis., 2007;13(0):626-34. Species: Mouse Sample Type: In Vivo Application: In vivo CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo. Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R J. Exp. Med., 2007;204(3):489-95. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The role of lymphoid tissue inducer cells in splenic white pulp development. Authors: Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ Eur. J. Immunol., 2007;37(11):3240-5. Species: Mouse Sample Type: Whole Cells Application: ICC An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG J. Exp. Med., 2006;203(12):2763-77. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y Blood, 2005;105(1):31-9. Species: Mouse Sample Type: Whole Cells Application: Neut Local delivery of recombinant vaccinia virus expressing secondary lymphoid chemokine (SLC) results in a CD4 T-cell dependent antitumor response. Authors: Flanagan K, Glover RT, Horig H, Yang W, Kaufman HL Vaccine, 2004;22(21):2894-903. Species: Mouse Sample Type: Cell Lysates Application: WB Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA J. Immunol., 2000;164(5):2650-9. Species: Rat Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL21/6Ckine Ser24-Gly133 Accession # P84444
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. Mature rat CCL21 shares 84% aa sequence identity with mouse CCL21.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion CC Chemokines and Receptors CCL21 & Tumor Immune Evasion Chemokines and Receptors Chemokines and Receptors in Angiogenesis Chemokines and Receptors in Tfh Cell Differentiation and Function Dendritic Cell Adhesion and Migration ICC / IHC Images: CCL21/6Ckine Neuroinflammation

参考图片

Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. Recombinant Mouse CCL21/6Ckine (Catalog # 457‑6C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457). The ND₅₀ is typically 0.5‑2.5 µg/mL.

CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.