Mouse CCL7/MARC Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL7/MARC Affinity Purified Polyclonal Ab (25 UG)

货号： AF-456-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-456-SP
别名	C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 7.5-45 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL7/MCP‑3/MARC.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6354 (Human); 20306 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection. Authors: Girkin J, Hatchwell L, Foster P, Johnston S, Bartlett N, Collison A, Mattes J J Immunol, 2015;194(10):4924-30. Species: Mouse Sample Type: In Vivo Application: Neut The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts. Authors: Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S , 2014;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity. Authors: Szymczak WA, Deepe GS J. Immunol., 2009;183(3):1964-74. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL7/MCP-3/MARC Gln24-Pro97 Accession # Q03366
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse MARC, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.
运输条件	Blue Ice
存放说明	4℃
参考文献	Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.

参考图片

Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody.

Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/
MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND₅₀ is typically 7.5-45 µg/mL.