Mouse CXCL2/MIP-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CXCL2/MIP-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-452-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-452-SP
别名	chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, and rrCINC-3 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses Sci Rep, 2016;6(0):37695. Species: Mouse Sample Type: In Vivo Application: In vivo TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression Sci Rep, 2016;6(0):38684. Species: Mouse Sample Type: Whole Cells Application: Bioassay Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses Sci Rep, 2016;6(0):37695. Species: Mouse Sample Type: Whole Cells Application: Bioassay Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K J Biol Chem, 2015;290(4):2053-68. Species: Mouse Sample Type: Whole Tissue Application: IHC The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis. Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F J Clin Invest, 2015;125(2):539-50. Species: Mouse Sample Type: Whole Cells Application: Neut Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways. Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D Am J Pathol, 2014;184(6):1877-89. Species: Mouse Sample Type: Whole Tissue Application: IHC CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S Int J Cancer, 2014;134(6):1346-58. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection. Authors: Stock A, Smith J, Carbone F J Exp Med, 2014;211(5):751-9. Species: Mouse Sample Type: Whole Cells Application: ICC Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns. Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM Infect. Immun., 2008;76(7):3001-10. Species: Mouse Sample Type: Whole Cells Application: Functional Assay The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation. Authors: Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K Crit. Care Med., 2007;35(9):2156-63. Species: Mouse Sample Type: In Vivo Application: In vivo CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Authors: Cardona AE, Gonzalez PA, Teale JM Infect. Immun., 2003;71(5):2634-42. Species: Mouse Sample Type: Whole Cells Application: ICC The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium. Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K J. Clin. Invest., 2001;108(9):1307-14. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CXCL2/MIP-2 Ala28-Asn100 Accession # P10889
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors Inflammatory Mediators Macrophage Activation Markers Molecules Secreted by VSMC Neuroinflammation

参考图片

Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/
MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452‑M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND₅₀ is typically 0.015‑0.075 µg/mL.

CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP‑2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.