Mouse CCL4/MIP-1 beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL4/MIP-1 beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF-451-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-451-SP
别名	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP‑1 beta and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha. In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10‑30 fold higher IgG concentration.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 1-15 µg/mL Neutralization: Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL4/MIP‑1 beta.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Authors: VE Thaney, AM ONeill, MM Hoefer, R Maung, AB Sanchez, M Kaul Sci Rep, 2017;7(0):46514. Species: Mouse Sample Type: Whole Cells Application: Neut Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N J. Immunol., 2008;181(9):6384-93. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A J. Exp. Med., 2008;205(9):2075-84. Species: Mouse Sample Type: In Vivo Application: In vivo CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses. Authors: Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL Diabetes, 2007;56(3):809-17. Species: Mouse Sample Type: In Vivo Application: In vivo CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Authors: Cardona AE, Gonzalez PA, Teale JM Infect. Immun., 2003;71(5):2634-42. Species: Mouse Sample Type: Whole Cells Application: ICC Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA J. Immunol., 2000;164(5):2650-9. Species: Rat Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL4/MIP-1 beta Ala24-Asn92 Accession # Q5QNV9
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455. Sun, X. et al. (2006) Infec. Immun. 74:5943. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821. Guan, E. et al. (2001) J. Biol. Chem. 276:12404. Guan, E. et al. (2002) J. Biol. Chem. 277:32348. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

参考图片

Detection of Mouse CCL4/MIP‑1 beta by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with
10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL4/MIP‑1 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CCL4/MIP‑1 beta in Mouse Small Intestine. CCL4/MIP‑1 beta was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Peyers patches. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Mouse CCL4/
MIP‑1 beta Antibody. Recombinant Mouse CCL4/MIP‑1 beta (Catalog # 451‑MB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL4/
MIP‑1 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA). The ND₅₀ is typically
0.2‑1.0 µg/mL.