| 货号 | AF431-SP |
| 别名 | CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP receptor; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4 | 全称 | Macrophage Stimulating Protein Receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse MSP R/Ron in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MSP R/Ron is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize MSP-induced chemotaxis in mouse peritoneal macrophages [Wang, M-H. et al. (1994) J. Biol. Chem. 269:3436]. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 50 ng/mL Recombinant Human MSP. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4486 (Human); 19882 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse MSP R/Ron Ser24-Arg960 (Ile372Leu) Accession # CAA52754 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Macrophage stimulating protein receptor (MSP R), encoded by the human RONand the mouse Stk,is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Mouse MSP R cDNA encodes a 1378 amino acid (aa) residue presursor protein with a 23 aa signal peptide, a 287 aa residue alpha chain (Ser 24 - Arg 310) and a 1068 aa residue transmembrane beta chain (Gly 311 - Thr 1378). Expression of MSP receptor is restricted to specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage. Both the heterodimeric MSP and the free MSP beta chain have been shown to bind to MSP R. However, only the heterodimeric MSP binding can induce receptor dimerization and phosphorylation and cause biological activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by MSP and Neutralization by Mouse MSP R/Ron Antibody. Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND50 is typically 0.05-0.2 µg/mL. |
