Mouse IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-400-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-400-SP
别名	Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
全称	Interleukin 1 alpha
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects mouse IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL‑1 alpha and recombinant cotton rat IL‑1 alpha is observed and less than 1% cross-reactivity with recombinant human IL‑1 alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.002-0.004 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3. Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D Proc Natl Acad Sci U S A, 2015;112(13):4050-5. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge. Authors: Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J PLoS Pathog, 2015;11(1):e1004625. Species: Mouse Sample Type: In Vivo Application: In vivo Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome. Authors: Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R J Biol Chem, 2014;289(51):35582-92. Species: Mouse Sample Type: Cell Lysates Application: WB Interleukin-1 deficiency prolongs ovarian lifespan in mice. Authors: Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y Proc Natl Acad Sci U S A, 2014;111(34):12492-7. Species: Mouse Sample Type: Tissue Homogenates Application: WB The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. Authors: Asrat S, Dugan A, Isberg R PLoS Pathog, 2014;10(7):e1004229. Species: Mouse Sample Type: Cell Lysates Application: WB IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN J. Immunol., 2011;187(9):4835-43. Species: Mouse Sample Type: Cell Lysates Application: WB Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, ONeill LA Nat. Immunol., 2010;11(10):897-904. Species: Mouse Sample Type: Cell Lysates Application: WB Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM Bone, 2007;40(5):1276-83. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1 Ser6-Ser161 Accession # Q62161
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Megakaryocytes Neuroinflammation Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

Detection of Recombinant Mouse and Rat IL‑1 alpha /IL‑1F1 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400‑ML), Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) and Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # 500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑400‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑1 alpha /IL‑1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

IL‑1 alpha /IL‑1F1 in Mouse Liver. IL‑1 alpha /IL‑1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF-400-NA). The ND₅₀ is typically 0.002-0.004 µg/mL in the presence of concanavalin A (1.25 µg/mL).