Mouse IFN-alpha/beta R1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IFN-alpha/beta R1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3039-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3039-SP
别名	alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFNBR; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1
全称	Interferon alpha/beta Receptor 1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) IFN‑ alpha / beta R2, rmIFN-gamma R1, and rmIFN-gamma R2 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ alpha / beta R1-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3454 (Human); 15975 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells. Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F Immunol. Lett., 2007;116(1):86-94. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1 Glu27-Thr429 Accession # P33896
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1-3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47-48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9-11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284. Takaoka, A. et al. (2000) Science 288:2357. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774. Lamken, P. et al. (2004) J. Mol. Biol. 341:303. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074. Yan, H. et al. (1996) EMBO J. 15:1064. Ragimbeau, J. et al. (2003) EMBO J. 22:537. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.

参考图片

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IFN‑ alpha / beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3039). The ND₅₀ is typically 1-4 µg/mL.