| 货号 | AF2765-SP |
| 别名 | carboxypeptidase A1 (pancreatic); carboxypeptidase A1; CPA; CPA1; EC 3.4.17; EC 3.4.17.1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CPA1 is observed and approximately 10% cross-reactivity with rhCPA2, rhCPB1, and recombinant mouse CPA4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Mouse Carboxypeptidase A1/CPA1 (0.25 µg/mL, Catalog # 2765‑ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is typically 2 µg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1357 (Human); 109697 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Carboxypeptidase A1/CPA1 Asn17-Tyr419 Accession # Q7TPZ8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of mouse CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified recombinant CPA1 corresponds to the pro form, which can be activated and assayed. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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