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货号： bs-1554R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1554R
- 英文名称
- Aquaporin 5
- 中文名称
- 水通道蛋白5抗体
- 别 名
- Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.
- Specific References (3) | bs-1554R has been referenced in 3 publications.[IF=1.09] Wang, Wei, et al. "Expression of AQP5 and AQP8 in human colorectal carcinoma and their clinical significance." World journal of surgical oncology 10.1 (2012): 1-5. IHC-P ; Human.PubMed:23148732[IF=4.25] Lim, Jae-Yol, et al. "Establishment and Characterization of Mesenchymal Stem Cell-like Clonal Stem Cells from Mouse Salivary Glands." Tissue Engineering (2014). Mouse.PubMed:25273691[IF=5.23] Yi, TacGhee, et al. "Single Cell Clones Purified from Human Parotid Glands Display Features of Multipotent Epitheliomesenchymal Stem Cells." Scientific Reports 6 (2016). FCM ; Human.PubMed:27824146
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 信号转导 通道蛋白 上皮细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 29kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse AQP5:201-265/265 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]
Function:
Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.
SWISS:
Q9WTY4
Gene ID:
11830
Database links:Entrez Gene: 362Human
Entrez Gene: 11830Mouse
Entrez Gene: 25241Rat
Omim: 600442Human
SwissProt: P55064Human
SwissProt: Q9WTY4Mouse
SwissProt: P47864Rat
Unigene: 298023Human
Unigene: 45580Mouse
Unigene: 10066Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
水通道蛋白(Aquaporin,AQP)是一组与水分子通透有关的特异性细胞膜转运蛋白。其中,AQP 5在哺乳动物气管、支气管的上皮细胞表达较常见.
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 5) Polyclonal Antibody, Unconjugated (bs-1554R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Aquaporin 5 Polyclonal Antibody, Unconjugated(bs-1554R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue).
Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1554R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: A549(blue).
Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.