Mouse IL-27 p28/IL-30 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-27 p28/IL-30 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1834-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1834-SP
别名	IL-30
全称	Interleukin 27 p28
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IL-27 p28/IL-30 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑27.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	246778 (Human); 246779 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes Sci Rep, 2016;6(0):30448. Species: Mouse Sample Type: Whole Cells Application: Neut Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Trypanosoma cruzi Infection Imparts a Regulatory Program in Dendritic Cells and T Cells via Galectin-1-Dependent Mechanisms. Authors: Poncini C, Ilarregui J, Batalla E, Engels S, Cerliani J, Cucher M, van Kooyk Y, Gonzalez-Cappa S, Rabinovich G J Immunol, 2015;195(7):3311-24. Species: Mouse Sample Type: Whole Cells Application: Neut IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis. Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM PLoS Pathog., 2012;8(7):e1002827. Species: Mouse Sample Type: Whole Cells Application: Neut Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: whole cells Application: Neut TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice. Authors: Kim HS, Go H, Akira S, Chung DH J. Immunol., 2011;187(8):4007-17. Species: Mouse Sample Type: In Vivo Application: In vivo Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10. Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA Nat. Immunol., 2009;10(9):981-91. Species: Mouse Sample Type: Whole Cells Application: Neut The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. Authors: Guo B, Chang EY, Cheng G J. Clin. Invest., 2008;118(5):1680-90. Species: Mouse Sample Type: Whole Cells Application: Neut Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H Immunity, 2008;29(1):68-78. Species: Mouse Sample Type: Whole Cells Application: Neut Interleukin-23 and interleukin-27 exert quite different antitumor and vaccine effects on poorly immunogenic melanoma. Authors: Oniki S, Nagai H, Horikawa T, Furukawa J, Belladonna ML, Yoshimoto T, Hara I, Nishigori C Cancer Res., 2006;66(12):6395-404. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30 Phe29-Ser234 Accession # Q8K3I6
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-27 p28 subunit (also known as IL-30 and Interleukin-27 subunit alpha) is a 26 kDa glycoprotein. IL-27 is a heterodimeric cytokine consisting of the IL-27 p28subunit that is an IL-12 p35 related protein and EBI3, which is relatedto IL-12 p40. The biological effects of IL-27 are mediated by theheteromeric receptor complex gp130 and TCCR/WSX-1.
运输条件	Blue Ice
存放说明	4℃
参考文献	ICC / IHC Images: IL-27 p28/IL-30 IL-12 Family Th1 Cells

参考图片

IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody. Recombinant Mouse IL‑27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL‑27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND₅₀ is typically 1‑5 µg/mL.

IL‑27 p28/IL‑30 in Mouse Thymus. IL‑27 p28/IL‑30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL‑27 p28/
IL‑30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.