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Mouse Notch-3 Affinity Purified Polyclonal Ab (25 UG)

货号: AF1308-SP 基本售价: 1378.1 元 规格: -

产品信息

概述
货号AF1308-SP
别名CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3
反应种属Mouse
应用Western Blot(0.1 µg/mL)
Flow Cytometry(2.5 µg/106cells)
Immunohistochemistry(5-15 µg/mL)
目标/特异性Detects mouse Notch‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human
Notch‑3 is observed and less than 5% cross-reactivity with recombinant rat (rr) Notch-1 and rrNotch-2 is observed.
使用方法Western Blot: 0.1 µg/mL
Flow Cytometry: 2.5 µg/106cells
Immunohistochemistry: 5-15 µg/mL
Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) to immobilized Recombinant Mouse Notch-3 (Catalog # 1308-NT) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
来源Polyclonal Goat IgG
产品组分
性能
供应商R&D Systems
Entrez Gene IDs4854 (Human); 18131 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Canonical notch signaling functions as a commitment switch in the epidermal lineage.
Authors: Blanpain C, Lowry WE, Pasolli HA, Fuchs E
Genes Dev., 2006;20(21):3022-35.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded

纯化方式Antigen Affinity-purified
免疫原S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Notch-3
Ala40-Glu468
Accession # Q61982
内毒素水平<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性Mouse
标记Unconjugated
溶解方法Reconstitute at 0.2 mg/mL in sterile PBS.
背景

Mouse Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2318 amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 20 aa transmembrane (TM) segment and a 655 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.

Notch-3 is predominantly expressed in the developing central nervous system of mice (2). Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Mouse Notch-3 shows 90% aa identity to human Notch-3 and 96% to rat Notch-3 over the entire protein.

运输条件Blue Ice
存放说明4℃
参考文献
  1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
  2. Lardelli, M. et al. (1994) Mech Dev. 46:123.
  3. Rebay, I. et al. (1991) Cell 67:687.
  4. Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
  5. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.