Mouse PDGF R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse PDGF R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF1062-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1062-SP
别名	alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
全称	Platelet-derived Growth Factor Receptor alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Spatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model Authors: I Tagge, A OConnor, P Chaudhary, J Pollaro, Y Berlow, M Chalupsky, D Bourdette, R Woltjer, M Johnson, W Rooney PLoS ONE, 2016;11(4):e0152480. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development Authors: K Noda, M Kitami, K Kitami, M Kaku, Y Komatsu Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development. Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G Cell Death Differ, 2015;22(2):351-63. Species: Mouse Sample Type: Whole Tissue Application: IF Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model. Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R Sci Rep, 2015;5(0):8468. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Hypoxia inducible factor-2alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors. Authors: Duan L, Takeda K, Fong G PLoS ONE, 2014;9(1):e84736. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis. Authors: Moore-Morris T, Guimaraes-Camboa N, Banerjee I, Zambon A, Kisseleva T, Velayoudon A, Stallcup W, Gu Y, Dalton N, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner D, Peterson K, Chen J, Evans S J Clin Invest, 2014;124(7):2921-34. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R Development, 2014;141(2):448-59. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Distinct fibroblast lineages determine dermal architecture in skin development and repair. Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Nature, 2013;504(7479):277-81. Species: Mouse Sample Type: tissue Application: IHC Frozen Abnormal development of NG2+PDGFR-alpha+ neural progenitor cells leads to neonatal hydrocephalus in a ciliopathy mouse model. Authors: Carter C, Vogel T, Zhang Q, Seo S, Swiderski R, Moninger T, Cassell M, Thedens D, Keppler-Noreuil K, Nopoulos P, Nishimura D, Searby C, Bugge K, Sheffield V Nat Med, 2012;18(12):1797-804. Species: Mouse Sample Type: Tissue Lysate Application: IP Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X J. Exp. Med., 2010;207(4):867-80. Species: Mouse Sample Type: In Vivo Application: In vivo Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke. Authors: Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA Nat. Med., 2008;14(7):731-7. Species: Mouse Sample Type: Cell Lysates Application: WB Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis. Authors: Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S J. Neuroimmunol., 2008;196(1):67-81. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N Clin. Cancer Res., 2006;12(9):2676-88. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis. Authors: Luyt K, Varadi A, Durant CF, Molnar E J. Neurochem., 2006;99(2):641-56. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala) Accession # P26618
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta bind PDGF with high affinity and are potent PDGF antagonists.
运输条件	Blue Ice
存放说明	4℃
参考文献	Betsholtz, C. et al. (2001) BioEssays 23:494. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

参考图片

PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND₅₀ is typically 1-5 µg/mL.