| 货号 | AF904-SP |
| 别名 | CNTN1; contactin 1; contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Contactin-1 in direct ELISAs. Detects human, mouse, and rat Contactin-1 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Contactin‑1-mediated adhesion of the C6 rat glioma cell line [Revest, J.M. et al. (1999) Adv. Exp. Med. Biol. 468:309]. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 2 µg/mL Recombinant Human Contactin‑1 Fc Chimera. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1272 (Human); 12805 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Contactin-1 Glu21-Ser993 Accession # CAA79696 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Contactin-1 (CNTN1) is a member of the contactin subgroup within the immunoglobulin superfamily. It was originally designated contactin in human, F3 in rodents, and F11 in chicken. Other members of this family in human include Contactin-2 (TAG-1), Contactin-5 (NB-2), and Contactin-6 (NB-3). Additional family members have been described in other species. CNTN1 shares less than 50% amino acid sequence identity with the other contactins. The human and rodent CNTN1 proteins share 96% sequence identity. The 998 amino acid mature protein contains 6 Ig-like domains and 4 fibronectin type III-like domains, and is attached to the membrane by a GPI anchor. CNTN1 is differentially expressed in numerous neuronal tissues and functions in nervous system development. It associates with two other cell-surface proteins believed to participate in signal transduction, receptor protein tyrosine phosphatase beta (RPTP beta ) and Contactin-associated protein (Caspr). Reported ligands include Nr-CAM and the extracellular matrix glycoprotein, tenascin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Contactin‑1 in Human Dorsal Root Ganglia. Contactin‑1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/ Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human, Mouse, and Rat Contactin‑1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human and Mouse Contactin‑1 by Simple WesternTM. Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑1 at approximately 158 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
Cell Adhesion Mediated by Contactin‑1 and Neutralization by Human Contactin‑1 Antibody. Recombinant Human Contactin‑1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Contactin‑1 Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904). The ND50 is typically 1-5 µg/mL. |
