Human IFN-beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IFN-beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF814-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF814-SP
别名	Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
全称	Interferon beta
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human IFN-beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IFN‑ beta 1, recombinant human (rh) IFN-alpha 2, rhIFN‑ gamma, recombinant cotton rat (rcr) IFN-alpha and rcrIFN‑ gamma is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.05-0.2 µg/mL in the presence of 10 ng/mL Recombinant Human IFN‑ beta.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3456 (Human); 15977 (Mouse); 24481 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. Authors: Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Kruger DH, Klempa B PLoS ONE, 2012;7(4):e35587. Species: Human Sample Type: Whole Cells Application: Neut Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C PLoS Pathog., 2011;7(4):e1002016. Species: Human Sample Type: Whole Cells Application: Neut The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes. Authors: Rascle A, Neumann T, Raschta AS, Neumann A, Heining E, Kastner J, Witzgall R Exp. Cell Res., 2009;315(1):76-96. Species: Human Sample Type: Whole Cells Application: Neut TLR3 can directly trigger apoptosis in human cancer cells. Authors: Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T J. Immunol., 2006;176(8):4894-901. Species: Human Sample Type: Whole Cells Application: Neut Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages. Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M J. Gen. Virol., 2004;85(0):2603-13. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IFN-beta
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Conventional/Classical Dendritic Cells Cytokines and Receptors in Angiogenesis IL-10/Interferon Family Infectious Diseases Plasmacytoid Dendritic Cells Th9 Cells

参考图片

IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody. Recombinant Human IFN‑ beta reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ beta (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND₅₀ is typically 0.05-0.2 µg/mL.