Human VCAM-1/CD106 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VCAM-1/CD106 Affinity Purified Polyclonal Ab (25 UG)

货号： AF809-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF809-SP
别名	CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1
全称	Vascular Cell Adhesion Molecule 1
反应种属	Human
应用	Western Blot(2 µg/mL)
目标/特异性	Detects VCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VCAM-1 is observed.
使用方法	Western Blot: 2 µg/mL Adhesion Blockade: The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human VCAM-1/CD106 Fc Chimera (Catalog # 862-VC, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
来源	Polyclonal Sheep IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7412 (Human); 22329 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. N-octanoyl-dopamine is an agonist at the capsaicin receptor TRPV1 and mitigates is chemia-induced acute kidney injury in rat. Authors: Tsagogiorgas C, Wedel J, Hottenrott M, Schneider M, Binzen U, Greffrath W, Treede R, Theisinger B, Theisinger S, Waldherr R, Kramer B, Thiel M, Schnuelle P, Yard B, Hoeger S PLoS ONE, 2012;7(8):e43525. Species: Human Sample Type: Cell Lysates Application: WB Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development Mesenchymal Stem Cells Transmigrate Between and Directly Through Tumor Necrosis Factor-alpha-Activated Endothelial Cells Via Both Leukocyte-Like and Novel Mechanisms. Authors: Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, Dvorak A, Karp J, Carman C Stem Cells, 2012;30(11):2472-86. Species: Human Sample Type: Whole Cells Application: ICC The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium. Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE Arthritis Rheum., 2007;56(6):1817-26. Species: Human Sample Type: Cell Lysates Application: WB Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Blood, 2006;107(6):2252-61. Species: Human Sample Type: Cell Lysates Application: WB A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. Authors: Carman CV, Springer TA J. Cell Biol., 2004;167(2):377-88. Species: Human Sample Type: Whole Cells Application: ICC A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. Authors: Carman CV, Springer TA J. Cell Biol., 2004;167(2):377-88. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line (CHO)-derived recombinant human VCAM-1 Extracellular domain
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha ₄beta₇. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha ₄beta₇ interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Myeloid Leukemia Adhesive Interactions in Allergy and Asthma Adipose-derived Stem Cell Markers Cancer Biomarkers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Endothelial Progenitor and Endothelial Cell Markers Hematopoietic Stem Cell Markers Human CD Marker Products ICC / IHC Images: VCAM-1/CD106 Immunoglobulin Superfamily CAMs Mesenchymal Stem Cell Markers Mini-review: VCAM-1/CD106 Mouse Tissue-Resident Macrophage Markers

参考图片

Detection of Human VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VCAM‑1/
CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VCAM‑1/CD106 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.