Human IGFBP-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IGFBP-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF804-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF804-SP
别名	BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4
全称	Insulin-like Growth Factor Binding Protein 4
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IGFBP‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGFBP-4 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-5 and rhIGFBP-6 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IGFBP‑4 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 12-60 µg/mL in the presence of 0.3 μg/mL Recombinant Human IGFBP‑4 and 14 ng/mL Recombinant Human IGF‑II.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3487 (Human); 16010 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells. Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K Reprod Biol Endocrinol, 2015;12(0):123. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction. Authors: Tchoukalova Y, Krishnapuram R, White U, Burk D, Fang X, Nijland M, Nathanielsz P Int J Obes (Lond), 2014;38(2):224-30. Species: Primate - Papio cynocephalus (Yellow Baboon) Sample Type: Whole Cells Application: WB The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A Gastroenterology, 2006;130(6):1754-63. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IGFBP‑4 Asp22-Glu258 Accession # AAA62670
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, ‑4, ‑5, ‑6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Jones, J. I. and D.R. Clemons (1995) Endocrine Rev. 16:3. Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619. Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.

参考图片

IGFBP‑4 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑4 Antibody. Recombinant Human IGFBP‑4 (Catalog # 804-GB) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑4 (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804). The ND₅₀ is typically 12‑60 µg/mL.

IGFBP‑4 in Human Placenta. IGFBP‑4 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGFBP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.