Human IL-33 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-33 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3625-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3625-SP
别名	C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
全称	Interleukin 33
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL‑33 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND₅₀) is typically 0.75-3.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	90865 (Human); 77125 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms. Authors: Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P J Clin Invest, 2015;125(7):2579-91. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis. Authors: Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T Clin. Exp. Allergy, 2012;42(2):218-28. Species: Human Sample Type: Whole Cells Application: Neut The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation. Authors: Byrne SN, Beaugie C, OSullivan C, Leighton S, Halliday GM Am. J. Pathol., 2011;179(1):211-22. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Authors: Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y Gut, 2010;59(4):531-41. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis. Authors: Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C Arthritis Rheum., 2009;60(3):738-49. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK J Korean Med Sci, 0;26(9):1132-9. Species: Human Sample Type: Synovial Fluid Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-33 Ser112-Thr270 Accession # O95760
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL-33 share 52‑58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins.
运输条件	Blue Ice
存放说明	4℃
参考文献	Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69. Schmitz, J. et al. (2005) Immunity 23:479. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323. Xu, D. et al. (1998) J. Exp. Med. 187:787. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930. Dinarello, C.A. (2005) Immunity 23:461. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

参考图片

Cell Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. In the presence of sub-optimal amounts of Hamster Anti‑Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recombinant Human IL‑33 (Catalog # 3625-IL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human IL‑33 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625). The ND₅₀ is typically 0.75-3.0 µg/mL.

IL‑33 in Human Tonsil. IL‑33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.