Human CCL5/RANTES Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CCL5/RANTES Affinity Purified Polyclonal Ab (25 UG)

货号： AF-278-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-278-SP
别名	CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G Clin Cancer Res, 2015;21(12):2840-50. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques. Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A J Immunol, 2014;193(1):277-84. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Tissue Application: IHC Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma. Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L Cancer Res, 2013;73(24):7254-64. Species: Mouse Sample Type: Whole Cells Application: Neut Expression and regulation of chemokines in murine and human type 1 diabetes. Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Diabetes, 2012;61(2):436-46. Species: Human Sample Type: Whole Cells Application: Flow Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype. Authors: Ouwehand K, Oosterhoff D, Breetveld M J. Invest. Dermatol., 2011;131(2):418-25. Species: Human Sample Type: Whole Tissue Application: Neut Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Human Sample Type: Whole Cells Application: Flow Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways. Authors: Renaud SJ, Sullivan R, Graham CH Placenta, 2009;30(4):313-9. Species: Human Sample Type: Whole Cells Application: Neut Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice. Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C Nat. Med., 2009;15(1):97-103. Species: Human Sample Type: Cell Lysates Application: IP Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS J. Immunol., 2009;182(6):3919-27. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation. Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA Am. J. Transplant., 2008;8(7):1512-22. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes. Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y J. Neurosci., 2008;28(13):3277-90. Species: Human Sample Type: Whole Cells Application: ICC Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA Nature, 2007;449(7162):557-63. Species: Human Sample Type: In Vivo Application: In vivo Renal transplantation: examination of the regulation of chemokine binding during acute rejection. Authors: Ali S, Malik G, Burns A, Robertson H, Kirby JA Transplantation, 2005;79(6):672-9. Species: Human Sample Type: Whole Cells Application: ICC The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells. Authors: Quandt J, Dorovini-Zis K J. Neuropathol. Exp. Neurol., 2004;63(4):350-62. Species: Human Sample Type: Whole Cells Application: ICC Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A J. Leukoc. Biol., 2003;73(5):584-90. Species: Human Sample Type: Whole Cells Application: Neut Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R Virology, 2002;294(1):209-21. Species: Human Sample Type: Whole Cells Application: Neut Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity. Authors: Sato E, Koyama S Eur. Respir. J., 2000;16(5):951-8. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL5/RANTES Ser24-Ser91 Accession # P13501
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Eosinophils Infectious Diseases Macrophage Activation Markers Molecules Secreted by VSMC Neuroinflammation Platelets

参考图片

CCL5/RANTES in Human PBMCs.

CCL5/RANTES was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (orange; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot. Western blot shows 25 ng of Recombinant Human CCL5/RANTES (Catalog # 278-RN), Recombinant Mouse CCL5/RANTES (Catalog # 478-MR), Recombinant Human CCL3/MIP‑1 alpha Isoform LD78a (Catalog # 270-LD), and Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Recombinant Human CCL5/RANTES (Catalog # 278‑RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA). The ND₅₀ is typically 0.1‑0.4 µg/mL.

CCL5/RANTES in Human Tonsil.

CCL5/RANTES was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.