Human IL-22 R alpha 1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-22 R alpha 1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2770-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2770-SP
别名	CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 receptor subunit alpha-1; IL22R; IL22R1; IL22RA1; IL-22RA1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
全称	Interleukin 22 Receptor
反应种属	Human
应用	Neutralization
目标/特异性	Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.
使用方法	Neutralization: Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	58985 (Human); 230828 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-22 contributes to TGF-beta1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells. Authors: Johnson J, Nishioka M, Chakir J, Risse P, Almaghlouth I, Bazarbashi A, Plante S, Martin J, Eidelman D, Hamid Q Respir Res, 2013;14(0):118. Species: Human Sample Type: Cell Lysates Application: WB IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients. Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S J Allergy Clin Immunol, 2013;131(2):562-70. Species: Human Sample Type: whole cells Application: Blocking IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K Eur. J. Immunol., 2009;39(10):2779-88. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1 Pro18-Thr228 Accession # Q8N6P7
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
运输条件	Blue Ice
存放说明	4℃
参考文献	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335. Boniface, K. et al. (2005) J. Immunol. 174:3695. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. Li, J. et al. (2004) Int. Immunopharmacol. 4:693. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047. Wolk, K. et al. (2004) Immunity 21:241. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

参考图片

IL‑10 secretion Induced by
IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody. Recombinant Human IL‑22 (Catalog # 782-IL) stimulates
IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ELISA Development Kit (Catalog # DY217B). IL‑10 secretion elicited by Recombinant Human IL‑22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑22 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND₅₀ is typically
0.2‑1 µg/mL.