| 货号 | AF-267-SP |
| 别名 | 3-nucleotidase; NT3; NT-3; NTF3 | 全称 | Neurotrophin 3 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry,Neutralization |
| 目标/特异性 | Detects human NT-3 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) NT‑4 is observed, and less than 1% cross-reactivity with rhBDNF is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB-BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human NT‑3. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4908 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3 Tyr139-Thr257 Accession # P20783 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer. The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. Recombinant Human NT‑3 (Catalog # 267-N3) stimulates proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT‑3 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human NT‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA). The ND50 is typically 0.1-0.4 µg/mL. | |
NT‑3 in Rat Brain. NT‑3 was detected in perfusion fixed frozen sections of rat brain (Purkinje cells in the cerebellum) using 10 µg/mL Human NT‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑267‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
