Human CXCL5/ENA-78 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CXCL5/ENA-78 Affinity Purified Polyclonal Ab (25 UG)

货号： AF254-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF254-SP
别名	CXCL5; ENA-78
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human CXCL5/ENA‑78 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) GCP-2 is observed and less than 5% cross-reactivity with recombinant mouse (rm) KC, recombinant rat CINC-1, rhGRO alpha, rhGRO gamma and rmGCP-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL5/ENA‑78-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 0.03 µg/mL Recombinant Human CXCL5/ENA‑78.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6374 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S Int J Cancer, 2014;134(6):1346-58. Species: Mouse Sample Type: Whole Tissue Application: IHC- Frozen Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer. Authors: Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ Am. J. Pathol., 2011;178(3):1340-9. Species: Human Sample Type: Whole Cells Application: Neut A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells. Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC Exp. Cell Res., 2008;314(11):2176-86. Species: Mouse Sample Type: Whole Cells Application: Neut Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients. Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D Int. J. Oncol., 2007;31(1):97-102. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM J. Immunol., 2002;169(11):6515-21. Species: Human Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CXCL5/ENA-78 Ala37-Asn114 Accession # P42830
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1‑3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35%‑51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3 - 6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6 - 9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593. Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250. Walz, A.. et al. (1991) J. Exp. Med. 174:1355. Strieter, R.M. et al. (1992) Immunol. Invest. 21:549. Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012. Begley, L.A. et al. (2008) Neoplasia 10:244. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226. Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465. Miyazaki, H. et al. (2006) Cancer Res. 66:4279. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421. Nufer, O. et al. (1999) Biochemistry 38:636. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545. Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219. Horton, L.W. et al. (2007) Cancer Res. 67:9791.

参考图片

Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78 Antibody. Recombinant Human CXCL5/ENA‑78 (Catalog # 254‑XB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL5/ENA‑78 (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254). The ND₅₀ is typically 0.2-1.0 µg/mL.

CXCL5/ENA‑78 in Human Breast. CXCL5/ENA‑78 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human
CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CXCL5/ENA‑78 in Human Breast Cancer Tissue. CXCL5/ENA‑78 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.