Human Legumain Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Legumain Affinity Purified Polyclonal Ab (25 UG)

货号： AF2199-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2199-SP
别名	AEP; Asparaginyl Endopeptidase; cysteine protease 1; legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34
反应种属	Human
应用	Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5641 (Human); 19141 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish. Authors: Ma, Liping, Shen, Yan-Qin, Khatri, Harsh P, Schachner, Melitta PLoS ONE, 2014;9(4):e95098. Species: Zebrafish Sample Type: Whole Tissue Application: IHC Fresh Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. Cell Res., 2012;22(9):1356-73. Species: Human Sample Type: Cell Lysates Application: WB Cystatin E/M suppresses legumain activity and invasion of human melanoma. Authors: Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R BMC Cancer, 2010;10(1):17. Species: Human Sample Type: Cell Lysates Application: WB Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD Atherosclerosis, 2008;0(0):. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase Ile18-Tyr433 Accession # Q99538
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha ₁ and thymosin alpha ₁₁, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by recombinant human (rh) Cystatin C and rhCystatin E/M and recombinant mouse Cystatin C (R&D Systems, Catalog # 1196‑PI, 1286-PI and 1238-PI, respectively).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, J.-M. et al. (1997) J. Biol. Chem. 272:8090. Tanaka, T. et al. (1996) Cytogenet. Cell Genet. 74:120. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194. Liu, C. et al. (2003) Cancer Res. 63: 2957. Li D.N. et al. (2003) J. Biol. Chem. 278:38980. Chen, J.M. et al. (2001) Biol. Chem. 382:777. Schwarz, G. et al. (2002) Biol. Chem. 383:1813. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

参考图片

Detection of Human Legumain/
Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of human kidney tissue, human heart tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Pro-Legumain/
Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.