| 货号 | AF2195-SP |
| 别名 | CA14; Carbonate dehydratase XIV; carbonic anhydrase XIVcarbonic anhydrase 14; carbonic dehydratase; CAXiV; CA-XIV; EC 4.2.1.1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CA1, rhCA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA13 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23632 (Human); 23831 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14 Gly19-Met290 Accession # Q9ULX7 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as anutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA14 is a type I membrane enzyme highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Carbonic Anhydrase XIV/CA14 by Western Blot. Western blot shows lysates of human and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
