货号 | AF2094-SP |
别名 | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353 |
反应种属 | Human/Mouse |
应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
目标/特异性 | Detects human and mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Semaphorin 6C is observed, 5% cross-reactivity with rhSemaphorin 6D is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, rhSemaphorin 6A, rhSemaphorin 7A, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, and rmSemaphorin 3F is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 10501 (Human); 20359 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Roles of semaphorin-6B and plexin-A2 in lamina-restricted projection of hippocampal mossy fibers. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6B Leu26-Ser603 Accession # Q9H3T3 |
生物活性 | Human, Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Semaphorin 6B (Sema6B) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑4). The four known Class 6 semaphorins are type I transmembrane glycoproteins with Sema domains but without other domains, making them most like Class 1 invertebrate semaphorins in structure (1‑4). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6B is expressed developmentally in subregions of the nervous system and muscle, and at low levels in most adult tissues (3, 4). Human Sema6B cDNA encodes a 25 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 20 aa transmembrane sequence and a 263 aa cytoplasmic portion. A cytoplasmic proline-rich sequence interacts with the SH3 domain of the c-src signaling protein (4). Full-length Sema6B is thought to form disulfide-linked homodimers (4). Alternate exon splicing creates a 492 aa (presumably) secreted form (Sema6B.1), and a 657 aa form with a shortened cytoplasmic tail (Sema6B.2) (3). Human Sema6B ECD shows 94%, 94%, 96% and 89% aa identity with corresponding mouse, rat, bovine and canine sequences, respectively. Crystal structures of semaphorins reveal that the 500 aa Sema domain forms an integrin-like seven-blade beta -propeller structure stabilized by 14 conserved cysteine residues (5). Sema6B is highly expressed in some glioblastoma and breast cancer cell lines. All-trans retinoic acid slows cancer cell growth and down‑regulates Sema6B expression, probably via dimerization with peroxisome proliferator-activated receptors (PPAR) that have a response element on the Sema6B gene (3, 6, 7). Semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors. Plexin-A4 binds Sema6A (high affinity) and 6B (low affinity) and mediates sympathetic ganglion axon-repulsion, independent of neuropilin-1 (8). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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