| 货号 | AF2050-SP |
| 别名 | CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein | 全称 | Melanoma Inhibitory Activity |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MIA in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8190 (Human); 12587 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intracellular sortilin expression pattern regulates proNGF-induced naturally occurring cell death during development. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MIA Gly25-Gln131 Accession # Q16674 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein(CD-RAP), is a secreted protein that plays an important role inmelanoma metastasis. It is highly expressed in malignant melanomas andis associated with tumor progression. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human MIA by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MIA at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
MIA in Human Melanoma. MIA was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
