Human/Mouse/Rat DEP-1/CD148 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat DEP-1/CD148 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1934-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1934-SP
别名	CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
全称	Density Enhanced Receptor Protein Tyrosine Phosphatase
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Immunoprecipitation(2.5 µg/500 µg cell lysate)
目标/特异性	Detects human, mouse, and rat DEP-1/CD148 in Western blots.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 2.5 µg/500 µg cell lysate
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5795 (Human); 19271 (Mouse); 29645 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M J Immunol, 2014;192(2):782-91. Species: Human Sample Type: Cell Lysate Application: WB Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling. Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP J. Biol. Chem., 2011;286(13):10918-29. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human DEP‑1/CD148 Arg997-Ala1337 Accession # Q12913
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta ‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain. Over aa 997-337, human Dep-1 shares 95% aa sequence identity with mouse and rat Dep-1.
运输条件	Blue Ice
存放说明	4℃
参考文献	Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680. Sorby, M. et al. (2001) Oncogene 20:5219. Jandt, E. et al. (2003) Oncogene 22:4175. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393. Borges, L.G. et al. (1996) Circ. Res. 79:570. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

参考图片

Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DEP‑1/CD148 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DEP‑1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.