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Human Fc gamma RII/CD32 Affinity Purified Polyclonal Ab (25 UG)

货号: AF1330-SP 基本售价: 1378.1 元 规格: -

产品信息

概述
货号AF1330-SP
别名CD32 antigen; CD32B; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIb; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b
全称Fc gamma Receptor II
反应种属Human
应用Western Blot(0.1 µg/mL)
Flow Cytometry(2.5 µg/106cells)
Immunocytochemistry(5-15 µg/mL)
目标/特异性Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Fc gamma RIIA is observed, and approximately 10% cross-reactivity with recombinant mouse Fc gamma RIIB is observed, and less than 1% cross-reactivity with rhFc gamma RIIIA is observed.
使用方法Western Blot: 0.1 µg/mL
Flow Cytometry: 2.5 µg/106cells
Blockade of Receptor-ligand Interaction: In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 2 μg/mL of human IgG to immobilized Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
Immunocytochemistry: 5-15 µg/mL
来源Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分
性能
供应商R&D Systems
Entrez Gene IDs2212 (Human); 14130 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Self double-stranded (ds)DNA induces IL-1beta production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies.
Authors: Shin M, Kang Y, Lee N, Wahl E, Kim S, Kang K, Lazova R, Kang I
J Immunol, 2013;190(4):1407-15.
Species: Human
Sample Type: whole cells
Application: Blocking
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.
Authors: Emmerich, Petra, Mika, Angela, Schmitz, Herbert
PLoS Negl Trop Dis, 2013;7(12):e2580.
Species: Human
Sample Type: protein
Application: WB
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia, 2011;54(10):2713-23.
Species: Human
Sample Type: Whole Cells
Application: Neut
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
J. Leukoc. Biol., 2008;84(4):1109-19.
Species: Human
Sample Type: Whole Cells
Application: Neut
C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
Atherosclerosis, 2007;196(1):129-35.
Species: Human
Sample Type: Whole Cells
Application: Neut

纯化方式Antigen Affinity-purified
免疫原Mouse myeloma cell line NS0-derived recombinant human Fc gamma RII/CD32
Ala46-Pro217
Accession # P31994
内毒素水平<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性Human
标记Unconjugated
溶解方法Reconstitute at 0.2 mg/mL in sterile PBS.
背景

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94‑99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

运输条件Blue Ice
存放说明4℃
参考文献
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
参考图片
Fc gamma  RII/CD32 in Human PBMCs. Fc gamma  RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma  RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.