| 货号 | AF1202-SP |
| 别名 | CST5; CSTD; cystatin 5; cystatin D; Cystatin-5; cystatin-D; cysteine-proteinase inhibitor; MGC71922 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C, rhCystatin SN, and rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin E/M and rhFetuin A is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Cystatin D (1 µg/mL, Catalog # 1202-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-Amc(100 µM, Catalog # ES009). The Neutralization Dose (ND50) is typically 13 µg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1473 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Cystatin D Thr29-Val142 Accession # CAA49838 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cystatin D is a member of family 2 of the cystatin superfamily. In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg-46 and Cys-46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and Cathepsins B, H, L and S. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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