Human IL-18 BPa Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-18 BPa Affinity Purified Polyclonal Ab (25 UG)

货号： AF119-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF119-SP
别名	IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa
全称	Interleukin 18 Binding Protein Isoform a
反应种属	Human
应用	Western Blot(0.5 µg/mL)
目标/特异性	Detects human IL‑18 BPa in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) IL‑18 BPc and rmIL-18 BPd is observed.
使用方法	Western Blot: 0.5 µg/mL Neutralization: Measured by its ability to neutralize IL‑18 BPa inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 0.1 µg/mL Recombinant Human IL‑18 BPa Fc Chimera, 40 ng/mL Recombinant Human IL‑18/IL‑1F4, and 20 ng/mL Recombinant Human TNF‑ alpha.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10068 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system. Authors: Sallmon H, Hoene V, Weber SC, Dame C J. Interferon Cytokine Res., 2010;30(2):55-8. Species: Human Sample Type: Cell Lysates Application: WB Generation and characterization of mice transgenic for human IL-18-binding protein isoform a. Authors: Fantuzzi G, Banda NK, Guthridge C, Vondracek A, Kim SH, Siegmund B, Azam T, Sennello JA, Dinarello CA, Arend WP J. Leukoc. Biol., 2003;74(5):889-96. Species: Human Sample Type: Serum Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa Thr31-Gly194 Accession # O95998
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
运输条件	Blue Ice
存放说明	4℃
参考文献	Mühl, H.et al. (2000) Biochem. Biophys. Res. Commun. 267:960. Kim, S-H.et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190. Calderara, S.et al. (2001) Virology 279:22.

参考图片

Detection of Human IL‑18 BPa by Western Blot.

Western blot shows conditioned media from HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285‑IF) and 40 ng/mL Recombinant Human TNF‑ alpha (Catalog #
210‑TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑18 BPa Inhibition of IL‑18/
IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Human IL‑18 BPa Antibody.

Recombinant Human IL‑18 BPa Fc Chimera (Catalog # 119-BP) inhibits Recombinant Human
IL‑18/IL‑1F4 induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Inhibition of Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) activity elicited by Recombinant Human IL‑18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND₅₀ is typically 1-5 µg/mL in the presence of Recombinant Human TNF‑ alpha (20 ng/mL).