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Human ErbB2/Her2 Affinity Purified Polyclonal Ab (25 UG)

货号: AF1129-SP 基本售价: 1378.1 元 规格: -

产品信息

概述
货号AF1129-SP
别名CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
全称Receptor Tyrosine Protein Kinase ErbB2
反应种属Human
应用Western Blot(0.1 µg/mL)
Flow Cytometry(2.5 µg/106cells)
Immunohistochemistry(5-15 µg/mL)
目标/特异性Detects human ErbB2/Her2 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse ErbB2 is observed and less than 5% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
使用方法Western Blot: 0.1 µg/mL
Flow Cytometry: 2.5 µg/106cells
Immunohistochemistry: 5-15 µg/mL
Inhibition of Cell Growth: Measured by its ability to inhibit proliferation in the SK‑BR‑3 human breast cancer cell line. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is typically 0.06-0.3 µg/mL.
来源Polyclonal Goat IgG
产品组分
性能
供应商R&D Systems
Entrez Gene IDs2064 (Human); 13866 (Mouse); 24337 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Novel method for differentiation between Trastuzumab and host adaptive response.
Authors: Denisova G, Jenkins K, Evelegh C, Bramson J
Mol. Immunol., 2011;48(15):1882-5.
Species: Human
Sample Type: recombinant HER-2
Application: WB
Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
Mol. Cancer Ther., 2008;7(10):3223-36.
Species: Human
Sample Type: Cell Lysates
Application: ELISA Development
FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival.
Authors: Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B
Cancer Res., 2008;68(7):2340-8.
Species: Human
Sample Type: Cell Lysates
Application: IP

纯化方式Antigen Affinity-purified
免疫原Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626
内毒素水平<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性Human
标记Unconjugated
溶解方法Reconstitute at 0.2 mg/mL in sterile PBS.
背景

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

运输条件Blue Ice
存放说明4℃
参考文献
  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rdet. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
参考图片
ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.