| 货号 | 46931S |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-YAP (Ser109) Antibody recognizes endogenous levels of YAP protein only when phosphorylated at Ser109. Based on sequence similarity, the antibody may recognize TAZ protein when phosphorylated at Ser66. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS1/2 kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (10). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
Western blot analysis of extracts from PANC-1 cells (human) and NIH/3T3 cells (mouse), untreated (-) or treated with λ phosphatase (+), using Phospho-YAP (Ser109) Antibody (upper) and YAP (D8H1X) XP® Rabbit mAb #14074 (lower). | |
Western blot analysis of extracts from various cell lines using using Phospho-YAP (Ser109) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). RL-7 cells are negative for YAP expression, confirming specificity of the antibody. | |
Immunoprecipitation of Phospho-YAP (Ser109) from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Phospho-YAP (Ser109) Antibody. Western blot analysis was performed using Phospho-YAP (Ser109) Antibody. |
