| 货号 | 3451S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Phospho-µ-Opioid Receptor (Ser375) Antibody detects transfected µ-opioid receptor only when phosphorylated at Ser375 of mouse MOR (or Ser377 of human MOR). |
| 使用方法 | WB(1:1000) IP (1:100) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides (1). Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase (1) and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades (2,3). Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance (4,5). As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains (6). Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization (7). |
| 存放说明 | -20C |
| 计算分子量 | 70 to 90 |
| 参考文献 | 1 . Law, P. Y. et al. (2000) Annu. Rev. Pharmacol. Toxicol. 40, 389-430. 2 . Polakiewicz, R. D. et al. (1998) J. Biol. Chem. 273, 12402-12406. 3 . Polakiewicz, R.D. et al. (1998) J Biol Chem 273, 23534-41. 4 . Finn, A.K. and Whistler, J.L. (2001) Neuron 32, 829-839. 5 . Kieffer, B.L. and Evans, C.J. (2002) Cell 108, 587-590. 6 . Yu, Y. et al. (1997) J. Biol. Chem. 272, 28869-28874. 7 . El Kouhen, R. et al. (2001) J. Biol. Chem. 276, 12774-12780. |
Western blot analysis of extracts from CHO cells, stably transfected with S375A mutant or Wild-type (WT) µ-opioid receptor, untreated or DAMGO-treated (1 mM for 5 minutes), using Phospho-µ-Opioid Receptor (Ser375) Antibody. Western blot分析CHO细胞，稳定转染S375A突变或野生型（WT）的µ-opioid receptor，未处理或1mM DAMGO处理5分钟，使用的抗体是Phospho-µ-Opioid Receptor (Ser375) Antibody 兔多抗。 | |
Western blot analysis of extracts from CHO cells, stably transfected with µ-opioid receptor, untreated or DAMGO-treated (1 mM) for various times, using Phospho-µ-Opioid Receptor (Ser375) Antibody. Western blot分析CHO细胞，稳定转染 µ-opioid receptor，未处理或1mM DAMGO处理多次，使用的抗体是Phospho- µ-Opioid Receptor (Ser375) Antibody 兔多抗。 | |
Confocal immunofluorescent analysis of normal mouse cerebellum labeled with Phospho-μ-Opioid Receptor (Ser375) Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析正常小鼠小脑，使用Phospho-μ-Opioid Receptor (Ser375) Antibody 兔多抗（红色）和CREB (86B10) Mouse mAb 鼠单抗 #9104 （绿色）进行标记。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 |
