Phospho-WIPI2 (Ser413) Antibody【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-WIPI2 (Ser413) Antibody

货号： 13571S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	13571S
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP
目标/特异性	Phospho-WIPI2 (Ser413) recognizes endogenous levels of WIPI2 protein only when phosphorylated at Ser413.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes.
存放说明	-20C
计算分子量	49
参考文献	1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88. 4 . Corvera, S. (2001) Traffic 2, 859-66. 5 . Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41. 6 . Krick, R. et al. (2006) FEBS Lett 580, 4632-8. 7 . Strømhaug, P.E. et al. (2004) Mol Biol Cell 15, 3553-66. 8 . Obara, K. et al. (2008) J Biol Chem 283, 23972-80. 9 . Jeffries, T.R. et al. (2004) Mol Biol Cell 15, 2652-63. 10 . Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404. 11 . Polson, H.E. et al. (2010) Autophagy 6, 506-22. 12 . Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404.

参考图片

Western blot analysis of extracts from Raji and KARPAS-299 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +) using Phospho-WIPI2 (Ser413) Antibody (upper) or total WIPI2 Antibody #8567 (lower).

Western blot 方法检测Raji和KARPAS-299细胞的提取物：未处理（-）或用TPA #4174处理（200nM，30分钟；+)，使用的抗体是Phospho-WIPI2 (Ser413) Antibody（上图）或 total WIPI2 Antibody #8567（下图）。

Western blot analysis of extracts from KARPAS-299 cells, treated with TPA #4174 (200 nM, 30 min) and then untreated (-) or λ phosphatase and calf intestinal phosphatase (CIP)-treated (+), using Phospho-WIPI2 (Ser413) Antibody (upper) or total WIPI2 Antibody #8567 (lower).

Western blot 方法检测KARPAS-299细胞提取物：首先用TPA #4174处理（200nM，30分钟）, 然后经未处理(-)或用λ 磷酸酶和小牛小肠磷酸酶(CIP)处理(+)，使用的抗体是Phospho-WIPI2 (Ser413) Antibody（上图）或WIPI2 Antibody #8567（下图）。

Immunoprecipitation of phospho-WIPI2 (Ser413) from KARPAS-299 cells treated with TPA #4174 (200 nM, 30 min) using Normal Rabbit IgG #2729 (lane 2) or Phospho-WIPI2 (Ser413) Antibody (lane 3). Lane 1 represents 10% input. Western blot was perform using Phospho-WIPI2 (Ser413) Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 was used as a secondary antibody.

免疫沉淀检测KARPAS-299细胞中phospho-WIPI2 (Ser413)的表达水平，TPA #4174处理（200nM，30分钟），使用的抗体是Normal Rabbit IgG #2729（泳道2）或Phospho-WIPI2 (Ser413) Antibody（泳道3）。泳道1代表10% input。利用Phospho-WIPI2 (Ser413) Antibody进行Western blot检测。鼠源 Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677作为二抗。