| 货号 | 2791S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 55-60 |
| 参考文献 | 1 . Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62. 2 . Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75. 3 . Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43. 4 . Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9. 5 . Vié, A. et al. (1999) J Biol Chem 274, 16788-95. 6 . Lindgren, N. et al. (2000) J Neurochem 74, 2470-7. 7 . Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93. 8 . Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51. 9 . Saraf, A. et al. (2007) J Biol Chem 282, 573-80. 10 . Kawahata, I. et al. (2015) Biochem Biophys Res Commun 465, 53-8. |
Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #2792 (lower). |
