| 货号 | 5267S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | Phospho-Stat4 (Tyr693) Antibody detects endogenous levels of Stat4 only when phosphorylated at Tyr693. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:25) |
| 供应商 | CST |
| 背景 | The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jerks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). |
| 存放说明 | -20C |
| 计算分子量 | 81 |
Western blot analysis of extracts from NK-92 cells, untreated or treated with Human Interleukin-2 (hIL-2) #8907 (2ng/ml, 15 minutes), using Phospho-Stat4 (Tyr693) Antibody (upper) or Stat4 (C46B10) Rabbit mAb #2653 (lower). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells starved of IL-2 overnight then treated with IL-12 (10ng/ml) for 4h and either 20 μl of Phospho-Stat4 (Tyr693) Antibody #5267 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
