| 货号 | 14865S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Members of the F-box family of proteins are characterized by the approximate 40 amino acid F-box motif named after cyclin F (1,2). F-box proteins constitute one of the four subunits of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. The substrate specificity of SCF complexes is determined by the interchangeable F-box proteins, which act as adaptors by associating with phosphorylated substrate proteins and recruiting them to the SCF core. F-box proteins contain two fundamental domains: the F-box motif mediates binding to Skp1 and a leucine rich repeat (LRR) domain mediates substrate interactions. |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing DDK-tagged full-length, human Skp2 wild-type protein (hSkp2-WT-DDK; +) or DDK-tagged full-length, human Skp2 S64A protein (hSkp2-S64A-DDK; +), using Phospho-Skp2 (Ser64) Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). | |
Immunoprecipitation of phosphorylated Skp2 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-Skp2 (Ser64) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Skp2 (Ser64) Antibody. | |
Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or Skp2 shRNA (shSkp2) using Phospho-Skp2 (Ser64) Antibody (upper), Skp2 (D3G5) XP®Rabbit mAb #2652 (middle), and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Western blot analysis of extracts from 293T and HeLa cells using Phospho-Skp2 (Ser64) Antibody. The phospho-specificity of the antibody was verified by pre-incubating the antibody with no peptide (left), Skp2 non-phosphopeptide (center), and Skp2 (Ser64) phosphopeptide (right). |
